Composite

Part:BBa_K4147008:Design

Designed by: Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-10-08)


PelB-(CBD)2-DrsB1 Construct with DsbA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1184
    Illegal NgoMIV site found at 1368
    Illegal AgeI site found at 392
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When adding a protein for co-expression, like in this case, make sure to put between the CDS and the signal peptide an RBS that allows independent transcription of each protein.


Source

This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct for the production of a recombinant Dermaseptin peptide in Nicotiana tabacum hairy roots with enhanced antimicrobial activity. On the other hand, the CDS for the disulfide interchange protein DsbA was obtained from the GenBank under the accession number NC_002516.2.

References